Saint Louis Encephalomyelitis

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J. F. Day2

 Culex nigripalpus mosquitoes are the most important disease vectors in Florida. They are the proven primary enzootic (normal level of virus transmission from mosquitoes to wild birds) and epidemic (unusually high level of virus transmission from mosquitoes to humans) vectors of St. Louis encephalitis SLE virus throughout the southern half of the state. In addition, they are likely involved in the transmission of eastern equine encephalitis (EEE) virus in Florida, from Titusville north to Jacksonville and west to Pensacola.

distribitionDistribution

 

Culex nigripalpus mosquitoes have a subtropical distribution. They are found in the southern United States, the Caribbean, Mexico, Central America and northern South America. In the USA, this species is found in warm, humid coastal habitats from North Carolina to Texas and up the Mississippi River basin as far north as Kentucky.

  

This species is most abundant in southern coastal regions where winter temperatures are mild. During warm periods, the species will expand its range northward, but adults and larvae are killed when they are exposed to extended periods of below freezing temperatures. In Florida, this species is found in all 67 counties. However, it is most abundant in the central portion of the peninsula from Brevard County south to Dade County, west across the state to Collier County and north along the Gulf of Mexico to Pensacola.

 

 CREDITS: Carpenter and LaCasse, 1955 edition of Mosquitoes of North America Figure 1. U.S. distribution of Culex nigripalpus.

 

 

Culex AdultDescription

Culex nigripalpus is a medium-sized mosquito. The proboscis is dark and the palpi short. The scutum is brown with fine dark bronze to brown scales. The pleura has few or no scales. Abdominal tergites are covered with dark-brown to black scales with bronze to metallic blue-green reflection. Narrow white basal bands are occasionally present on the dorsal surface of some abdominal segments. Legs are dark with reflective bronze to metallic blue-green scales. The posterior surface of the femora and tibiae are pale. In general, this is a dark brown, nondescript species with no obvious distinguishing characteristics, making field identification to the species level extremely difficult.

 

CREDITS: Carpenter and LaCasse, 1955 edition of Mosquitoes of North America Figure 2. Culex nigripalpus adult.

 

Culex AdultLife Cycle

Gravid Culex nigripalpus females take flight during evening crepuscular periods and search throughout the night for oviposition sites. Eggs of this species have been reported in virtually every type of aquatic habitat. However, the species is best described as a flood water Culex. Females prefer to lay eggs in freshly flooded roadside ditches and agricultural furrows, habitats that flood infrequently and, once flooded, remain wet for 10 to 14 days before drying down completely. Eggs are cemented together loosely and laid in rafts containing from 90 to 210 eggs. They hatch 24 to 36 hours after being laid. CREDITS: Scott Bauer, USDA Figure 3. Adult female Florida SLE mosquito, Culex (Culex) nigripalpus Theobald, with blood meal.

 

Larvae develop in the rich organic mixture found in shallow flooded ditches. Larval development time is temperature dependent, and is most rapid in mid-summer when the water temperature in the ditches may exceed 100 degrees F. Larvae develop through four instars, each lasting approximately 24 hours in summer and 48 hours in winter. Fourth instar larvae molt to form pupae from which the adult mosquito emerges 36 to 48 hours later.

 

Culex Larvae

Adults mate near the breeding site and females begin host seeking when they are approximately 48 hours old. Females are extremely opportunistic in their choice of a host, feeding on vertebrates ranging from tree frogs to humans. Females seek hosts and blood feed mainly during the crepuscular periods before sunrise and after sunset. Host selection is influenced by the relative abundance of hosts found in the mosquito’s foraging habitat. During dry winter and spring months, females host seek primarily in lush, humid forest floors and canopies where they usually encounter and feed on resting birds. During wet periods in the summer and autumn, females can forage in open fields and in other habitats from which they were previously excluded due to the dry conditions. In these open habitats, female mosquitoes locate and blood feed on mammalian species such as horses, cows, dogs, rabbits, armadillos and humans.

 

Once blood is obtained, it takes the mosquito about four days to digest the meal (again, this is temperature dependent with faster digestion occurring during the hot summer months) and form a batch of eggs. Once eggs are formed, they are laid as soon as a suitable oviposition site is located.

 

CREDITS: Peggy Greb, USDA Figure 4. Larvae of Culex (Culex) nigripalpus Theobald.

 

 

Medical Significance

Culex nigripalpus is the most important disease vector in Florida where it is the predominant vector of SLE virus and a minor vector of EEE virus. During all of the major Florida SLE epidemics; 1959, 1961 and 1962 in the Tampa Bay area and 1977 and 1990 in the central region of the state, Culex nigripalpus proved to be the only mosquito vector of consequence relative to human SLE transmission. Most of the EEE virus isolates from mosquitoes have been from Culiseta melanura (Coquillett), the known enzootic vector of the EEE virus to wild birds. In addition, a large number of EEE virus isolates in Florida have come from Cx. nigripalpus females.

 

One reason this species is an efficient arboviral vector is due to its “egg dumping” behavior. Because females prefer to lay their eggs in freshly flooded ditches, there are often long periods, especially during summer droughts, when there are few available oviposition sites. During these periods, the number of gravid females builds steadily, sometimes accounting for up to 90% of the adult female population. After a rainfall sufficient to flood roadside and agricultural ditches (usually 2 or more inches in 24 hours), gravid females oviposit quickly, seek hosts and blood feed in a cycle synchronized by the rainfall event. If the period of drought preceding the rainfall was sufficiently long to allow infected mosquitoes time to complete the extrinsic incubation of the SLE virus (10 to 14 days), then many of the mosquitoes will be infective and able to transmit SLE virus during the blood meal following oviposition. The shear abundance of this species in south Florida (it is found from urban parks to rural farms throughout the year), along with its egg dumping and mass blood feeding behaviors, make it an exceptionally dangerous mosquito species relative to arboviral transmission.

 

Surveillance and Managment of Culex nigripalpus

Due to the medical significance of this species, vector surveillance, with the ultimate goal of predicting human cases of SLE virus, is of critical importance. Historically, New Jersey light traps and CDC miniature light traps have been used to monitor the abundance of Cx. nigripalpus adults. However, we have found that resting collections made with ground aspirators, provide a superior measure of Cx. nigripalpus adult populations. This is because males as well as females in all gonotrophic conditions (host seeking, blood fed, partially blood fed and gravid) are taken in aspirator collections. Due to their green color, newly emerged adults of both sexes can be identified and counted, thus providing an accurate measure of adult emergence.

 

Because Cx. nigripalpus females prefer to lay their eggs in freshly flooded ditches, huge numbers of adults are often observed during the wet summer months in south Florida. The vast number of breeding habitats in which this species is found results in adult populations that are difficult to regulate and thus, this species is seldom targeted for control efforts. A notable exception occurs during periods of known or suspected SLE transmission. These transmission cycles are usually identified by increases in sentinel chicken or wild bird antibody seroconversions to SLE virus or by the occurrence of confirmed human SLE cases. During SLE epidemics, control efforts directed against Cx. nigripalpus females are sometimes undertaken. Because of the overwhelming number of oviposition sites, larval control is seldom attempted. Ultra Low Volume (ULV) adulticide applications of malathion, naled or fenthion by ground vehicles can be directed against Cx. nigripalpus adults, especially in areas such as hospitals and retirement villages, where humans that are more susceptible to SLE infection reside. Occasionally, ULV applications of adulticides are made from the air. Because of the expense, when county-wide broadcast applications of aerial ULV treatments are attempted, an effort is sometimes made to coordinate aerial insecticide applications with the rainfall-driven oviposition cycles of gravid females. This technique maximizes the kill of older gravid and blood seeking females that are most capable of transmitting the SLE virus. To date, there is little evidence that ULV adulticide applications, made from the ground or from the air, control Cx. nigripalpus females or interrupt SLE transmission cycles in Florida.

 

Selected References

Cutwa MM, O’Meara GF. (Unknown). Identification guide to common mosquitoes of Florida. http://fmel.ifas.ufl.edu/Key/index.htm (23 July 2004).

 

Dame D, Fasulo TR. (2003). Mosquitoes. Public Health Pesticide Applicator Training Manual. http://vector.ifas.ufl.edu/ (21 January 2004).

 

Day, J.F. 1991. Epidemic proportions. Natural History. July, 50-53

 

Day, J.F. and G.A. Curtis. 1993. Annual emergence patterns of Culex nigripalpus females before, during and after a widespread St. Louis encephalitis epidemic in south Florida. Journal of American Mosquito Control Association 9:249-255.

 

Day, J.F. and G.A. Curtis. 1994. When it rains, they soar–and that makes Culex nigripalpus a dangerous mosquito. American Entomologist 40:162-167.

 

Koehler, P.G., D.E.Short and T.R. Fasulo. (1998). Pests In and Around the Home . UF/IFAS, CD-ROM.

 

How Can the Risk of Exposure be Reduced?

Vaccines for humans are not currently available for the vast majority of arthropod-borne pathogens including WNV and SLEV. Pay attention to medical and veterinary alerts and follow the specific recommendations. Medical alerts will be posted on the FMEL Encephalitis Information System at http://eis.ifas.ufl.edu.

The best method of reducing risk of exposure is to avoid mosquito bites through personal protection by wearing mosquito repellents and protective clothing during times when mosquitoes are most active.

 

Personal Protection

Personal protection against biting arthropods, particularly when they are infected with dangerous pathogens, remains one of the most important ways to avoid disease. Avoid mosquitoes. Make sure screens are in good repair to prevent mosquitoes from entering homes. If you must enter areas where there is a threat of encountering infected mosquitoes, wear protective clothing.

Finally, use a personal insect repellent that provides a reasonable Complete Protection Time (CPT). The CPT is the total time following repellent application that the treated individual will remain bite free. For example, under normal conditions the CPT for a 5% formulation of DEET (diethyl toluamide, presently the most effective insect repellent) is approximately 2 hours. The CPT for a 24% DEET formulation is more than 4 hours. For more information on repellents, refer to the University of Florida/IFAS Fact Sheet ENY-671 “Mosquito Repellents”.

• Avoid exposure to mosquitoes – stay indoors during peak biting time.

• If you must be outside during peak biting time, wear long sleeves and pants.

• Wear mosquito repellents when outside during peak biting time. Use mosquito repellents containing DEET. Be sure to follow the directions on the label

• Make sure window and door screens are in good repair to prevent mosquitoes from entering homes.

• Remove unnecessary sources of water outside the home that may provide breeding places for mosquitoes.

• Flush out the water in bird baths and outdoor pet dishes every 3 – 4 days.

• Remove leaf litter, standing water and debris from roof gutters and boat covers.

• For more information see: The Florida Medical Entomology Lab’s Fact Sheet on Personal Protection (ENY-671 – Mosquito Repellents)

 

Public Protection

Fortunately, the USA has some of the best mosquito and arthropod control programs in the world. Vector control and personal protection against vectors and the diseases they carry are the best way to avoid infection with vector-borne pathogens. Strategies that might be effective against Eastern Equine Encephalitis infection include: source reduction of mosquito breeding sites; focal applications of insecticides directed against adult and immature mosquitoes; Public Service Announcements to educate residents about the vector, the disease, and disease avoidance; tips to help prevent home-invasion by infected vectors; and information about the most effective means of personal protection. St. Louis and West Nile encephalitis epidemics and outbreaks in Florida have proved that an effective means of reducing human infection is to widely disseminate accurate information through the media to educate the public. An individual’s first line of defense during a vector-borne disease emergency is knowledge and personal protection.

 

Further information on mosquito-borne viruses in Florida

For updates on the current situation of mosquito-borne diseases in Florida, visit the Florida Medical Entomology Laboratory’s Encephalitis Information System to view current health alerts (http://eis.ifas.ufl.edu). Additionally, information is provided to assist readers in understanding the real risk of exposure to the mosquito-borne viruses in Florida.

Originally posted 2011-06-09 22:01:09. Republished by Blog Post Promoter

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